Isolated, characterization and identification of bacterial strains associated with rice culture
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Abstract
The rice (Oryza sativa L.) is the cereal of more demand in the country. A favorable strategy to increase its yields is the biofertilizers application. During the years 2015 and 2016 were isolated a total of twenty-eight bacterial strains associated to this species. For it, they took samples of rizospheric soils and plants of three cultivars (´INCA LP-5´, ´INCA 30-19´ and ´Perla de Cuba´), in two regions of the Mayabeque province. Its employed two soils types, commonly used in their culture. For the isolations the culture medium was used (Nutrient Agar, LGI, Ashby y NFB), with the purpose the obtaining of bacterial strains of the genus Bacillus, Gluconacetobacter, Azotobacter and Azospirillum. Among these genus exists diversity of the microorganism endophytic, rhizospheric and associative of free live. Of them, seven were discarded because their morphological and physiologic characteristics didn't belong together with none of the four genus in study. Among 21 remaining, twenty fix atmospheric nitrogen, eleven solubilizes phosphorous and fourteen they produce siderophore. Two strains like Azotobacter vinalandii were characterized, two as Azospirillum brasilense and seventeen of Bacillus, although this aspect should be confronted with technical of molecular biology in later studies. The characterization of the metabolic potential of this microorganisms, will allow advancing in the evaluation of the stimulation of the growth of the same ones, with the purpose of selecting the most efficient to formulate a bioproduct dedicated to the benefit of this cultivation.
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