Morfofisiologic and biologic characterization of Metarhizium spp. cuban isolates promising for the Cylas formicarius Fabricius control
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Abstract
The entomopathogenic fungi Metarhizium anisopliae is one of the most used for the biological control of agriculture pest in Cuba. Nowadays the research´s are applies to the isolation of new strains, of good climatic conditions adaptation and therefore get a better control of target pest. The objective of this work was studied morphological, cultural and physiological characteristics of seven new isolates of Metarhizium spp. with pathogenic activities against Cylas formicarius Fabricius. The isolates were described take into account colonies morphology and conidia measurements. The fungus growth rates and sporulation level was calculated at different culture medium. Also the growth at different temperatures (28, 30, 32, 34 and 37ºC) was determinates. The isolates showed pathogenic activities against C. formicarius and was demonstrated that are included in the M. anisopliae species complex considering the descriptions obtained. These results indicated that best temperature range for the growth of the cultures was 28-30ºC. The isolates LBM-5 and LBM-10 showed the highest growth rates at the temperatures and culture medium evaluated. The highest conidia concentration was showed by LBM-5 and LBM-267 in Complete Media, Saboraud´s Dextrose Agar and Malt Extract Agar.
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