Isolate, revitalize and evaluate species of cellulolytic fungus for their use in the agricultural biotechnology
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Abstract
In this work were possible to isolate, revitalize and evaluate species of fungus with high cellulolytic activity, conforming a work bank for their potential use in the agricultural biotechnology and the compost production. 25 strains of fungus were isolated, gathered starting from cellulosic remains (cloth, cardboard and paper) and from soil in different INIFAT’s area (compost area, forest and Cuartón of Roig). Of these strains, 18 were identified in species: Ascotricha bosei, Ascotricha xylina, Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, Curvularia lunata, Chaetomium globosum, Chaetomium indicum, Dycima vesiculifera, Memnoniella echinata (2), Memnoniella levispora, bicolor Stachylidium, Stachybotrys niragirica, Trichoderma hamatum, Trichoderma viride, Trichoderma harzianum and seven in genus: Curvularia sp., Cladosporium sp., Chaetomium sp., Fusarium sp., Penicillium sp., Stachybotrys sp. and Trichoderma sp.; according to the morphologic description of their colonies. Also12 strains conserved in the Collection 853 of the World Federation of Microbial Cultivations (WFCC) of the INIFAT, during more than 15 years; reported as cellulolytic, were revitalized and reidentified, as well as five belonging to the group of the Collection of Edible and Medicinal Strains. The cellulolytic activity was evaluated by the qualitative techniques of carboximetilcelulosa (CMC) and filter paper (exoglucanasas assay and loss weight) to select the strains with more potenciality: Ascotricha bosei, Aspergillus niger, Chaetomium globosum, Memnoniella echinata, Stachybotrys sp., Stachybotrys niragirica, Trichoderma harzianum, Trichoderma viride and Pleurotus ostreatus. Finally all were conserved in tubes with a half of Malt- Agar (MA) medium immersed in 15 mL of Slight Liquid Petrolatum, replied in number of ten.
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