Viability and recovery of viable plants of a garlic cryopreserved sample
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Abstract
Investigations on garlic cryopreservation developed at the Institute for Fundamental Research on Tropical Agriculture (INIFAT) targets to preserve garlic clones adapted to tropical climate. The implementation of a cryopreserved collection requires determining the predicted number of viable explants in a genebank accession. That quantity can be estimated based on the number of explants processed, viability level of the control, and a desired confidence level. The garlic control cryopreserved sample evaluated in this assay included 40 apices and was cryopreserved by vitrification protocol, with the PVS3 solution. The sample had a viability of 0.76 and regenerated 23 viable plants. That number of plants was comparable to the quantity estimated for that viability level and number of processed explants, considering a confidence level of 95 % (25 plants). These results confirm the fitness of vitrification protocol, with the PVS3 solution, for garlic cryopreservation at INIFAT’s genebank, and constitute a contribution to the research for the implementation of a garlic cryopreserved collection in Cuba.
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References
Acker, J.P.; Adkins, S.; Alves, A.; Horna, D. y Toll, J. (2017). Feasibility study for a safety back-up cropreservation facility. Independent expert report: July 2017. Rome (Italy): Biodiversity International. 100 p. ISBN: 978-92-9255-073-8.
Bettoni, J.C.; Bonnart, R. y Volk, G.M. (2020). Challenges in implementing plant shoot tip cryopreservation technologies. Plant Cell, Tissue and Organ Culture (PCTOC) https://doi.org/10.1007/s11240-020-01846-x (consultado: septiembre 2020).
Dussert, S.; Engelmann, F. y Noirot, M. (2003). Development of probabilistic tools to assist in the establishment and management of cryopreserved plant germplasm collections. CryoLetters, 24: 149-160. PMID 12908025.
Ellis, D.; Skogerboe, D.; Andre, C.; Hellier, B. y Volk, G. (2006). Implementation of garlic cryopreservation techniques in the National Plant Germplasm System. CryoLetters 27 (2): 99-106. PMID16799441
Ellis, D. (2019). Plant cryobanks-How will genebank managers 100 year from now judge what we are doing today? Cryobiology, 91:158. DOI: 10.1016/j.cryobiol.2019.10.055 (Fecha de consulta: agosto del 2020).
FAO (2020). Institutional Information. SDG Indicator 2.5.1: Number of plant and animal genetic resources for food and agriculture secured in either medium or long-term conservation facilities. Disponible en: www.fao.SDG megadata. March 2020. (Fecha de consulta: 21 de agosto de 2020).
Jenderek, M.M. y Reed, B.M. (2017). Cryopreserved storage of clonal germplasm in the USDA National Plant Germplasm System. In Vitro Cell. Dev. Biol. Plant., DOI: 10.1007/s11627-017- 9828-3.
Keller, E.R.J.; Stavělíková, H.; Zámečník, J.; Kotlińska, T.; Kik, C.; Esnault, F.; Solberg S. y Miccolis, V. (2012). EURALLIVEG: Establishment of a European core collection by cryopreservation and virus elimination in garlic. En: T. Wako (ed), Proc. 6th IS on Edible Alliaceae. Acta Hort., 969, ISHS 20: 319-327. DOI:10.17660/Acta Hortic.2012.969.41 (Fecha de consulta: enero 2019).
Keller, E.R.J. y Senula, A. (2013). Micropropagation and cryopreservation of garlic (Allium sativum L.). En: Maurizio Lambardi et al. (eds.), Protocols for Micropropagation of Selected Economically-Important Horticultural Plants, Methods in Molecular Biology, 994. DOI:10.1007/978-1-62703-074-8_28. (Fecha de consulta: septiembre 2019).
Keller, E.R.J. y Senula, A. (2016). Recent aspects of Allium cryopreservation in the federal German genebank. En: Gokce A.F. (ed.). Proc. VII Int. Sym. on Edible Alliaceae. Acta Hortic, ISHS 1143: 35-44. DOI 10.17660/ActaHortic. 2016.1143.6.
Keller, E.R.J. (2005). Improvement of cryopreservation results in garlic using low temperature preculture and high-quality in vitro plantlets. CryoLetters, Nov- Dec; 26 (6): 357-366. PMID 16547550.
Keller, E.R.J.; Grübe, M.; Hajirezaei, M.R.; Melzer, M.; Mock, H.P.; Rolletschek, H.; Senula, A. y Subbarayan, K. (2016). Experience in large-scale cryopreservation and links to applied research for safe storage of plant germplasm. Acta Hortic., 1113, 239-250. DOI:10.17660/ActaHortic.2016.1113.36.
Keller, E.R.J. y Kik, C. (2018). Allium Genetic Resources. 23-52 En: M. Shigyo et al. (eds.). The Allium Genomes, Compendium of Plant Genomes. 217 pp. DOI:10.1007/978-3-319-95825-5_3.
Kim, H.H., Popova, E., Shin, D.J.; Yi, J.Y.; Kim, C.H.; Lee, J.S.; Yoon, M.K. y Engelmann, F. (2012). Cryobanking of Korean Allium germplasm collections: Results from a 10 years’ experience. CryoLetters, 33 (1): 45-57. PMID 22434122.
Linsmaier, E.M. y Skoog, F. (1965). Organic growth factor requirements of tobacco tissue cultures. Physiologia Plantarum, 18: 100-127. https://doi.org.10.1111/j.1399- 3054.1965.tb06874.x.
Liu, X.X.; Wen, Y.B.; Cheng, Z.H. y Mou, S.W. (2017). Establishment of a garlic cryopreservation protocol for shoot apices from adventitious buds in vitro. Scientia Horticulturae, 226: 10–18. DOI: 10.1016/j.scientia.2017.08.016
Liu, X.X.; Mou, S.W. y Cheng, Z.H. (2019). Effects of cryopreservation on plant growth, bulb characteristics and virus reduction of garlic (Allium sativum L.). Cryolettters, 2019 Nov-Dec; 40 (&): 322-332. PMID 33966058.
Murashige, T. y Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. DOI:10.1111/j.1399-3054 1962.tb08052.
Nagakubo, T.; Nagasawa A. y Ohkawa H. (1993). Micropropagation of garlic through in vitro bulblet formation. Plant Cell, Tissue and Organ Culture, 32: 175-183. https://doi.org/10.1007/BF00029840.
Panis, B. (2019). Sixty years of plant cryopreservation: from freezing hardy mulberry twigs to establishing reference crop collections for future generations. Acta Hortic., 1234. ISHS 2019. Proc. III International Symposium on Plant Cryopreservation. K. Thammasiri et al. (eds). DOI: 10.17660/ActaHortic.2019.1234.1 (Fecha de consulta: mayo 2021).
Pritchard, H.W. (2018). The rise of plant cryobiotechnology and demise of plant cryopreservation? Abstracts Cryobiology, 85, 160–161. Disponible en https://doi.org/10.1016/j.cryobiol.2018.10.1 59 (Fecha de consulta: 8 de septiembre de 2020).
Reed, B.M. (2017). Plant cryopreservation: a continuing requirement for food and ecosystem security. In Vitro Cell. Dev. Biol. Plant., 53(4): 285-288. e-ISBN 978-0-387-72276-4. DOI: 10.1007/s11627-017-9851-4. (Fecha de consulta: enero 2019).
Torres, M.A. (2012). Desarrollo de la conservación in vitro del germoplasma de ajo (Allium sativum L.). Informe final del proyecto. Programa Nacional Mejoramiento Vegetal y Recursos Fitogenéticos (01500148). Instituto de Investigaciones Fundamentales en Agricultura Tropical (INIFAT), La Habana.
Volk, G.M.; Henk, A.D.; Jenderek, M.M. y Richards, C.M. (2016). Probabilistic viability calculations for cryopreserving vegetatively propagated collections in genebanks. Genet. Resour. Crop Evol., 64:1613-1622. DOI:10.1007/s10722-016-0460-6. (Fecha de consulta: noviembre de 2017).
Walters, C. y Pence, V.C. (2020). The unique role of seed banking and cryobiotechnologies in plant conservation. Plants, People, Planet., 3(1): 83-91. https://doi.org/10.1002/ppp3.10121 (Fecha de consulta: mayo de 2021).
Wang, M.R.; Chen, L.; Zhang, Z.; Blystad, D.R. y Wang, Q.C. (2018). Cryotherapy: a novel method for virus eradication in economically important plant species. In: Loyola-Vargas, V.; Ochoa-Alejo, N. (eds.) Plant Cell Culture Protocols. Methods in Molecular Biology, 1825:257-268. Humana Press, New York, N.Y. https://doi.org/10.1007/978-1-4939-8594-4_17 (Fecha de consulta: enero de 2020).
Wang, M.R.; Lambardi, M.; Engelmann, F.; Pathirana, R.; Panis, B.; Volk, G.M. y Wang, Q.Ch. (2020). Advances in cryopreservation of in vitro-derived propagules: technologies and explant sources. Plant Cell, Tissue and Organ Culture (PCTOC), 144, 7-20. https://doi.org/10.1007/s11240-020-01770-0. (Fecha de consulta: agosto de 2020).