Effect of control pest against microorganism with agriculture potential
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Abstract
The use of biofertilizers with control pest is a challenge for Cuba and the world. With the aim of improving this kind of research, was determined the no toxic effect over bacteria growth of active substances clotanidin, imidacloprid y it’s mixed with tiocarb, against Rhizobium, Mesorhizobium, Bradyrhizobium, Azotobacter, Azospirillum, Gluconacetobacter y Bacillus strains under in vitro conditions. This was determinate by Zonal Box Diffusion Method. The substance fludioxonil and the combination tiametoxan, fludioxonil/mefenoxan and difenoconazo, imidacloprid and tebuconazol (Celest, Celest Top, Apron Star and Yunta) had moderated inhibitory effect. Thiram and its join with imidacloprid and pencycuron (Gaucho MT y TMTD) have a high toxicity. In spite of Rhizobium, Azotobacter, Bacillus megatherium and Bacillus subtilis strains are present in black bean (Phaseolus vulgaris) with the most aggressive product between 106 and 109 UFCxseed-1 without difference with Poncho, Gaucho FS, Celest y Celest Top, cupper and Thrichoderma harzianum. This result and the same behavior in greenhouse condition of black bean (Phaseolus vulgaris) seed with these products and Rhizobium showed the possibility to combine biofertilizers and control pest for the integral management of crops. Special attention deserves Poncho and Celest Top, due to the damage make in nodulation of black bean. It was similar to cupper.
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References
Afzal, A y Bano, A. (2008). Rhizobium and Phosphate Solubilizing Bacteria Improve the Yield and Phosphorus Uptake in Wheat (Triticum aestivum). International Journal of Agriculture and Biology. 10 (1): 85-88.
Beneduzi, A., Peres, D., Vargas, L.X., Bodanese-Zanettini., M.H y Passaglia, L.P. (2008). Evaluation of genetic diversity and plant growth promoting activities of nitrogen-fixing bacilli isolated from rice fields in South Brazil. Applied Soil Ecology. 39: 311– 320.
BIOCEN. (2001). Manual de Medios de Cultivo. La Habana. Centro Nacional de Biopreparados. BIOCEN.
Charpentier, M y Oldroyd, G. (2010). How close are we to nitrogen-fixing cereals?. Current Opinion in Plant Biology.10 (13):556–564.
Dibut, B., Martínez. R., Ríos. Y y Ortega, M. (2003). DIMARGON-M, nueva variante nutritiva para la producción de biofertilizantes y bioestimuladores a base de Azotobacter. En: Resúmenes del V Encuentro de Agricultura Orgánica, La Habana, 36pp.
Dibut, B., R. Martínez Viera., M. Ortega, Y. Ríos y L. Fey. (2010). Obtención de un biofertilizante mixto de amplio espectro de acción. Efecto sobre el cultivo de la rosa (Rosa spp.). Agrotecnia de Cuba 34 (1): 2010.
Döbereiner, J., Reis, V.M., Paula. M.A y Olivares, F. (1993). Endophitic diazotroph in sugarcane, cereals and tuber plants. En: New Horizons in Nitrogen Fixation. R.Palacio., J. Mora y W.E. Newton, Eds. Klumer Academic Publisher, Netherlands. 671–676.
Kavanagh, F.W. (1974). Métodos para evitar errores en el ensayo microbiológico de antibióticos. J. Pharm. Sci. 63. (9). 1459.
Madhaiyan, M, Poonguzhali, S, Hari, K, Saravanan, VS, Sa, TM. (2006). Influence of pesticides on the growth rate and plant growth promoting traits of Gluconacetobacter diazotrophicus. Pest Biochem Physiol 84: 143–154.
Mafia, R.G., Alfenas, A.C., Maffia, L.A., Ferreira, E.M., Binoti, D.H.B y G.M Ventura Mafia. (2009).Plant growth promoting rhizobacteria as agents in the biocontrol of eucalyptus mini-cutting rot. Tropical Plant Pathology. 34. (1) : 010-017.
Martínez, R., López. M., Dibut, B., Parra, C y Rodríguez, J. (2007). La fijación biológica de nitrógeno atmosférico en condiciones tropicales. Venezuela. MPPAT. 172 pp.
Martínez, V. R; López, M; Brossard, F. M; Tejeda, G. G; Pereira, A. H; Parra, Z. C; Rodríguez, S. J y Alba, A. (2006). Procedimientos para el estudio y fabricación de Biofertilizantes Bacterianos. Ed. INIA - Maracay. Venezuela, 88 pp.
Ríos, Y., Ortega, M., Tejeda, G y Rodríguez, J. (2011). Caracterización de cepas de Bacillus subtillis con potencial para la elaboración de productos biológicos. Agrotecnia de Cuba 35 (2).
Rivera, D., Germán, E., Obando, M., Bonilla R y Camelo, M. (2010). Efecto de diferentes plaguicidas sobre el crecimiento de Azotobacter chroococcum. Revista Colombiana de Biotecnología.7:1. 94-102.
Rodríguez, J. (2010). “Formulación de un bioproducto mixto a partir de Azotobacter chroococcum y Bacillus subtilis para el tratamiento de semillas de tomate (Solanum lycopersicum L.)”. Tesis para optar por el Grado Científico de Máster en Agricultura Urbana. 81 pp.
Saravanan., V.S., Madhaiyan, M., Osborne, J., Thangaraju, M y Sa, T.M. (2008). Occurrence of Gluconacetobacter diazotrophicus and nitrogen fixing Acetobacteraceae members. Their possible role in plant growth promotion. Microbial Ecology. 55: 130-140.
Tejeda, G, G; Rodríguez, S. J; García, G. R; Martínez, V. R; Castellanos, J. J; Dibut, A. B; Ríos, R. Y; Gutiérrez, H. L; Arozarena, D. N; Plana, P. L; Izquierdo, D. L; García, H. A; Ortega, G. M; Croche, A. G; Socas, E. U; Mesa, V. E; Simanca, M. M.E; Fraga, R. S y Fey, G. L. (2008). Obtención de biofertilizantes bacterianos a partir de Bacillus subtilis. Convención Internacional Trópico´2008. III Congreso de Agricultura Tropical.
Velásquez-Hernández, M. L; Baizabal-Aguirre, V.M; Cruz-Vázquez, F; Trejo-Contreras, M.J; Fuentes- Ramírez, L. Bravo-Patiño, A; Cajero-Juárez, A y Chávez-Moctezuma, M.P; Valdez-Alarcón, J.J. (2010). Gluconacetobacter diazotrophicus levansucrase is envolved in tolerance to NaCl, sucrose and desiccation, and in biofilm formation. Arch Microbiol. DOI 10.1007/s00203-010-0651-z. Publisher online: 20 de noviembre del 2010.
Yang, S., Tang, F., Gao, M., Krishnan, H.B y H. Zhu. (2010). R gene-controlled host specificity in the legume– rhizobia symbiosis. PNAS. 107. ( 43): 18735–18740.
