Evaluación de somaclones de tomate (Solanum lycopersicum L.) obtenidos a partir del cv. ´Cuba C-2781’ mediante las técnicas de selección in vitro y variación somaclonal
Article Sidebar
Main Article Content
Abstract
The present work was carried out with the objective to evaluate, in field, the behaviour to early blight and the agronomics and morphologic characteristics to eighteen somaclones of tomato obtained by in vitro selection from cv. ‘Cuba C- 2781’ in the Tissue Culture Laboratory of INIFAT. The experiment was conducted in Organopónico ‘Van Troi’ from Boyeros, Ciudad de La Habana during September/05 to February/06. There were evaluated 8 characters and with the results were realized a principal components analysis (ACP) and a cluster analysis using a Euclidean distance metric by StatGraphics Program version 5,0. The ACP showed that 3 components have extracted the 79,3 % of the variability and the variables fruit average weight, total of fruit number y soluble solid content showed the higher weights by components (1, 2 y 3 respectively). Cluster analysis created 3 clusters, the cluster I with 8 members (42,1 %), the cluster II with 7 members (36,8 %) and the cluster III with 4 members (21,1 %) among them the original cultivar ‘Cuba C- 2781’ (control). The results indicated that was possible to obtaine 15 somaclones (78,93 %) different of original cultivar. It demonstrated the effectively of biotechnological techniques in order to induce a new genetic variability in this specie.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Those authors who have publications with this journal accept the following terms of the License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0):
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
The journal is not responsible for the opinions and concepts expressed in the works, they are the sole responsibility of the authors. The Editor, with the assistance of the Editorial Committee, reserves the right to suggest or request advisable or necessary modifications. They are accepted to publish original scientific papers, research results of interest that have not been published or sent to another journal for the same purpose.
The mention of trademarks of equipment, instruments or specific materials is for identification purposes, and there is no promotional commitment in relation to them, neither by the authors nor by the publisher.
How to Cite
References
Álvarez M y Torres V (1993): Análisis de correlaciones de variedades y líneas de tomate (Lycopersicon esculentum Mill). Cultivos Tropicales, 5 (1): 49- 59.
Al-Zahim MA, Ford-Lloyd BV y Newbury HJ (1999): Detection of somaclonal variation in garlic (Allium sativum L.) using RAPD and cytological analysis. Plant Cell Rep., 18: 473-477.
Capote A, González-Chávez M, Peteira B, Rodríguez N, Pérez, O y Marrero N (2004): Selección in vitro y caracterización de líneas de tomate (Lycopersicon esculentum) resistentes a Alternaria solani. Memorias BioVeg’03. Ciego de Avila, ISSN
Carvalho JB, de Brito L, Boiteux L y Alberto M (1994): Cultivo do tomate (Lycopersicon esculentum Mill.) para industrilização. Centro Nacional de Pesquisas de Hortaliças (CNPH), 36 pág.
García RL, Veitía N, Dita MA, Bermúdez Y, Clavelo J, Romero C y García L (1998): Empleo del cultivo de tejidos y la mutagénesis in vitro para la mejora de resistencia a Alternaria solani en papa (Solanum tuberosum L.) var. ‘Desirée’. Resúmenes III Encuentro Latinoamericano de Biotecnología Vegetal, 1-5 Junio, La Habana, pág. 203.
Hernández MM, Kowalski B, Lorenzo P y Ortiz U (1991): Efectividad del empleo de filtrados de Alternaria solani Ellis y Martin (Jones y Grout) en la selección in vitro de formas resistentes en papa (Solanum tuberosum L.). Cultivos Tropicales, 12(2): 48 – 50.
Ingram DS y MacDonald MV (1985): Selection of mutants in vitro: Text of lecture. International Symposium on Nuclear Techniques and in vitro Culture for Plant Improvement. Vienna, Austria, 19-23 August.
GNAU (2000): Manual Técnico de Organopónico y Huertos Intensivos. Agrinfor, 145 pág.
Miller JC y Tanksley SD (1990): RFLP analysis of phylogenetic relationship and genetic variation in the genus Lycopersicon. Theor. Appl. Genet., 80: 437 – 448.
Murashige T. y Skoog F. (1962): A revised medium for rapid growth and bioassays tobacco tissue culture. Physiol. Plant., 15: 473- 497.
Nuez F (1995): El cultivo del tomate. Madrid, Ediciones Mundi Prensa, 793 pág.
Peralta IE, Knapp S y Spooner DM (2005): New species of wild tomatoes (Solanum section Lycopersicon: Solanaceae) from northern Peru. Systematic Botany, 30 (2): 424- 434.
Pratta G, Zorzoli R y Picardi LA (1996): Evaluación de caracteres de interés agronómico en especies del género Lycopersicon. Horticultura Argentina, 15 (3): 25- 32.
Sheppard SKL y Sohndal MR (1986): Selection for early blight disease resistance in tomato: Use of tissue culture with Alternaria solana culture filtrate. 6th Int. Congr. Plant Tissue and Cell Culture. Abstr. Univ. Minnesota, Minneapolis, E.U., pág. 211.