Endosymbiotic population asociated to native spore of Glomeromycetes in two layers of soil in one tropical forest
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Abstract
Up to now, still remain unknown the physiological function of spore’s endosymbiotic bacteria from arbuscular mycorrhizal fungi (AMF). Neither is defined their way of transmission. Since earthworm’s casting (EWC) are regarded as truly “hot spots of biodiversity”. We purposed to balance the populations of three functional groups of endosymbiotic bacteria (FGEB) to spores of AMF, amongst the EWC and the nearby soil (NS), in order to relate them with a supposed way of horizontal transmission. We collect five samples during the dry season in a secondary forest from the Biosphere’s Reserve, Sierra del Rosario, Cuba’s western. We assess the spore’s biovolume and the mycomass of AMF external mycelium. Also, we quantified the endosymbiotic populations of total’s heterotrophic, diazotrophic and phosphate solubilizer’s bacteria as well. We found that FGEB had highest populations in the NS. Phosphate solubilizer’s bacteria were smaller than total’s heterotrophic and diazotrophic populations within both soil’s layers, in that order. Despite our results do not confirm, and neither reject the mycostatic phenomenon, we’re convinced to the presence of such endosymbiotic community. Furthermore, we are almost sure of a tight interdependence within bacteria, plants and AMF. According with our design we refuse the horizontal’s transmission hypothesis, which would indicate a supposed higher population of endosymbiontic bacteria inside the spores of EWC. Results achieved about functional groups of endosymbiotic bacteria on both soil’s layer; encourage us towards new challenges to follow studying the physiology of such endosymbiotic microorganisms.
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References
Anderson, J. M. y P. W. Flanagan (1989): Biological processes regulating organic matter dynamics in tropical soils. En: Dynamic of Soil Organic Matter in Tropical Ecosystems (D. C. Coleman, J. M. Oades & G. Uehara, eds.). University of Hawaii Press, Honolulu, Hawaii.
Andrade, G., Mihara, K. L., Linderman R. G. y G. J. Bethlenfalvay (1998): Soil aggregation status and rhizobacteria in the mycorrhizosphere. Plant and Soil 202:89-96.
Artursson, V., Finlay, R.D., y J. K. Jansson (2006): Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth. Environ. Microbiol. 8(1): 1-10.
Azcón, R. (1987): Germination and hyphal growth of Glomus mosseae in vitro: effects of rhizosphere bacteria and cell-free culture media. Soil Biol Biochem 19: 417–419.
Bago, B., Pfeffer, P.E. y Y. Shachar-Hill (2000): Carbon metabolism and transport in arbuscular mycorrhizas. Plant Physiol. 124: 949-957.
Bago, B., Pfeffer, P.E. y Y. Shachar-Hill (2004): Metabolismo del Carbono y su Regulación en Hongos Formadores de Micorrizas Arbusculares. En: Avance en el Conocimiento de la Biología de las Micorrizas. (eds. J.T. F. Hernández, V.O. Portugal, R. F. Cerrato), Universidad de Guanajuato, Guanajuato: 29-43.
Barea, J.M. (1997): Mycorrhiza-bacteria interactions on plant growth promotion. En: Plant Growth Promoting Rhizobacteria. Ogoshi, A., Kobayashi, K., Homma, Y., Kodama, F., Kondo, N., and Akino, S. (eds). Paris, France: OECD Press, pp. 150–158.
Barea, J.M., Azcón-Aguilar, C., y R. Azcón (1997): Interactions between mycorrhizal fungi and rhizosphere microorganisms within the context of sustainable soil-plant systems. En: Multitrophic Interactions in Terrestrial Systems. Gange, A.C., and Brown, V.K. (eds). Oxford, UK: Blackwell Science, pp. 65–77.
Bécard, G., Doner, L.W., Rolin, D.B., Douds, D.D. y P.E. Pfeffer (1991): Identification and quantification of trehalose in vesicular-arbuscular mycorrhizal fungi by in-vivo 13C NMR and HPLC analyses. New Phytol. 118: 547-552.
Bianciotto, V., Bandi, C., Minerdi, D., Sironi, M., Tichy, H.V., y P. Bonfante (1996 a): An obligately endosymbiotic mycorrhizal fungus itself harbors obligately intracellular bacteria. Appl. Environ. Microbiol. 62: 3005-3010.
Bianciotto, V., Minerdi, D., Perotto, S., y P. Bonfante (1996 b): Cellular interactions between arbuscular mycorrhizal fungi and rhizosphere bacteria. Protoplasma 193: 123-131.
Bianciotto, V., y P. Bonfante (2002): Arbuscular mycorrhizal fungi: a specialised niche for rhizospheric and endocellular bacteria. Antonie Van Leeuwenhoek 81: 365–371.
Bianciotto, V., Genre, A., Jargeat, P., Lumini, E., Bécard, G. y P. Bonfante (2004): Vertical transmission of endobacteria in the arbuscular mycorrhizal fungus Gigaspora margarita through generation of vegetative spores. Appl. Environ. Microbiol. 70: 3600-3608.
Bonfante, P., Balestrini, R., y K. Mendgen (1994): Storage and secretion processes in the spore of Gigaspora margarita Baecker & Hall as revealed by high-pressure freezing and free substitution. New Phytol 128: 93–101.
Bonkowski M., Griffiths B. S. y K. Ritz (2000): Food preferences of earthworms for soil fungi. Pedobiologia 44, 666-676.
Bouché M. B. (1975): Action de la faune sur les etats de la matiere organique dans les ecosystemes. En: Kilbertius G, Reisinger O, Mourey A & da Fonseca J A C (ed.) Humification et biodegradation. Pierron, Sarreguemines. pp. 157-168.
Brown, G.G. (1995): How do earthworms affect microfloral and faunal community diversity? Plant and Soil. 170: 209-231.
De Boer,W., Verheggen, P., Klein Gunnewiek, P.J.A., Kowalchuk, G.A., y J.A. van Veen (2003): Microbial community composition affects soil fungistasis. Appl. and Environ. Microbiol. 69: 835-844.
Declerck, S., Strullu, D.G. y C. Plenchette. (1998): Monoxenic culture of the intraradical forms of Glomus sp. Isolated from a tropical ecosystems: A proposed methodology for germplasm collection. Mycologia- 90: 579-585.
Fitter, A.H. (1987): An architectural approach to the comparative ecology of plant root systems. New Phytol. 106: 61-67.
Fragoso, C., P. Lavelle, E. Blanchart, B. K. Senapati, J. J. Jimenez, M. A. Martinez, T. Decaëns y J. Tondoh (1999): Earthworm Communities of Tropical Agroecosystems: Origin, Structure and Influence of Management Practices. En: Earthworm Management in Tropical Agroecosystems (P. Lavelle, L. Brussard & P. Hendrix, eds.), CAB International Publishing, 300 pp.
Frobisher, M. (1969): Microbiología. Editorial Academia León. España.
Gange, A. (1993): Translocation of mycorrhizal fungi by earthworms during early succession. Soil Biology and Biochemestry, 25: 1021-1026.
Gerdemann, J. W. y T. H. Nicolson (1963): Spores of mycorrhizal Endogone species extracted from soil by wet-sieving and decanting. Transactions of the British Mycological Society 46: 235-244.
Giovannetti, M. y B. Mosse (1980): An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytol. 84: 489-500.
Harinikumar, K. M., Bagyaraj, D. J., y R. D. Kale (1991): Vesicular arbuscular mycorrhizal propagules in earthworm cast. En: Advances in Management and Conservation of Soil Fauna. (G.K. Veeresh, D. Rajagopal and C. A. Viraktamath eds.), Oxford and IBH, New Delhi: 605-610.
Hepper, C.M. (1989): Isolation and culture of VA mycorrhizal (VAM) fungi. En: VA Mycorrhiza, eds. C.L. Powell D.J. Bagyaraj. CRC Press, Boca Raton, 95-112.
Herrera R.A., E. Furrazola, R.L. Ferrer, R. Fernández y Y. Torres (2004): Functional strategies of root hairs and arbuscular mycorrhizae in an evergreen tropical forest, Sierra del Rosario, Cuba. Revista CENIC Ciencias Biológicas. 35(3): 113-123.
Hijri, M., Redecker, D., MacDonald-Comber Petetot, J.A., Voigt, K., Wöstemeyer, J., y I.R. Sanders (2002): Identification and Isolation of two Ascomycete fungi from spores of the arbuscular mycorrhizal fungus Scutellospora castanea. Applied and Envoronmental Microbiol. 68(9): 4567-4573.
Kim, K.Y., Jordan, D., y G.A. McDonald (1998): Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizae on tomato growth and soil microbial activity. Biol Fert Soils 26: 79–87.
Jargeat, P., Cosseau, C., Ola’h, B., Jauneau, A., Bonfante, P., Batut, J., y G. Bécard (2004): Isolation, free-livimg capacities and genome structure of “Candidatus Glomeribacter gigasporarum”, the endocellular bacterium of the mycorrhizal fungus Gigaspora margarita. J. bacterial. 186: 8676-6884.
Lavelle, P. (1983): The structure of earthworm communities. En: Earthworm Ecology: From Darwin to Vermiculture. (J. E. Satchell ed.). Chapman & Hall, London: 449-465.
Lavelle P. (1984): The Soil System in the Humid Tropics. Biol. International 9, 2-17.
Lee, K. K., M. V. Reddy, S. P. Wani & N. Trimurtulu (1996): Vesicular-arbuscular mycorrhizal fungi in earthworm casts and surrounding soil in relation to soil management of a semi-arid tropical Alfisol. Applied Soil Ecology, 3: 177-181.
Linderman, R.G. (1997): Vesicular-arbuscular mycorrhizal (VAM) fungi. En: The Mycota. Caroll, G.C., and Tudzynski, P. (eds). Berlin, Germany: Springer-Verlag, pp. 117–128.
Lockwood, J. L. (1977): Fungistasis in soils. Biol. Rev., 52: 1-43.
McGrady, M. H. (1918): Tables for rapid interpretation of fermentation tube’s results. Can. Pub. Health. J., (9): 275-286.
Meyer, J.R. y R.G. Linderman (1986): Response of subterranean clover to dual inoculation with vesicular- arbuscular fungi and a plant growth-promoting bacterium, Pseudomonas putida. Soil Biol Biochem. 18: 185-190.
Minerdi, D., Fani, R., Gallo, R., Boarino, A., y P. Bonfante (2001): Nitrogen fixation genes in an endosymbiotic Burkholderia strain. Appl. Environ Microbiol., 67: 725-732.
Morton, J.B. y G.L. Benny (1990): Revised classification of arbuscular mycorrhizal fungi (Zygomycetes): a new order, Glomales, two new suborders, Glomineae and Gigasporineae, and two new families, Acaulosporaceae and Gigasporaceae, with an emendation of Glomaceae. . Mycotaxon, 37:471-491.
Pan, B. y J. K. Vessey (2001): Response of the endophytic diazotroph Gluconacetobacter diazotrophicus on solid media to changes in atmospheric partial O2 pressure. Appl. and Environ. Microbiol. 67 (10): 4694-4700.
Pfeffer, P.E., Douds, D.D, Bécard, G., y Y. Shachar- Hill (1999): Carbon uptake and the metabolism and transport of lipids in arbuscular mycorrhiza. Plant Phys. 120: 587-598.
Rambelli A. (1973): The Rhizosphere of mycorrhizae. En: Ectomycorrhizae. Marks, G.L. and Koslowski, T.T. (Eds.), Academic Press, New York, pp. 299–343.
Ramos, A. y V. Callao (1967): El empleo de la solubilización de fosfatos en placas como teoría diferencial bacteriana. Microbiol. Española, 20(1), 10-15.
Reddell, P. y A. V. Spain (1991): Earthworms as vectors of viable propagules of mycorrhizal fungi. Soil Biology and Biochemistry, 23(4): 767-774.
Reiss, S. y A. Rambelli (1980): Preliminary notes o mycorrhizae in a natural tropical rain forest. En: Tropical Mycorrhiza Research. Mikola, P. (ed.), Claredon Press Oxford, New York, 143-145.
Rillig, C. M., S. F. Wright, M. F. Allen y C. B. Field (1999): Rise in carbon dioxide change soil structure. Nature, 400- 628.
Sieverding, E. (1991): Vesicular-Arbuscular Mycorhiza Management in Tropical Agrosystems. Schriftenreihe der GTZ, No. 224, Deutsche Gesellschaft für Technische Zusammenarbeit, GTZ, 371 p.
Tiwari, S. C. y R.R. Mishra (1993): Fungal abundance and diversity in earthworm casts and uningested soil. Biology and Fertility of Soil, 16: 131-134.
Truffen F., Eason W. R. y J. Scullion (2002): The effect of earthworms and arbuscular mycorrhizal fungi on growth of and 32P transfer between Allium porrum plants. Soil Biol. & Biochem., 34, 1027-1036.
Watanabe, I., y W. L. Barraquio (1979): Low levels of fixed nitrogen required for isolation of free living organism from rice roots. Nature (227), 565-566.
Wurst S., Dugassa-Gobena D., Langel R., Bonkowski, M., y S. Scheu (2004): Combined effects of earthworms and vesicular-arbuscular micorrizas on plant and aphid performance. New Phytol., 163, 169-176.