Slow growth conservation of garlic (Allium sativum L.) germplasm
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Abstract
Garlic is a specie with high culinary value and medicinal properties. Its medicinal activity has been recognized until ancient times, but more recently, its action over human health has got the interest of researchers world wide. Garlic does not produce botanic seeds under standard cultivation conditions. It’s germplasm is conserved as field collections, and bulb storage between vegetative growth cycles, so it is exposed to different natural and human risks. Slow growth conserved collections reduce the risk of germplasm loss. The objective of this paper is to evaluate slow growth garlic germplasm conservation and acclimation at INIFAT’s Gene Bank; and bulb characteristics after two cycles of sowings: the first in an orchard (organoponic) and the second under field conditions. Conservation temperature of 5- 6oC reduced plant growth, expressed as leaf development and bulbification lower rate. Bulbs harvested from the orchard were conserved in order to use them as “seeds” for field evaluation. Field harvested bulbs maintained phenotypical characteristic and recovered bulb proportions. The number seeds (cloves) harvested from field sowing overcame acclimation loses with a multiplication rate of 20 to 30 seed bulbs, depending on clone. Even so these results could be optimised¸ they are a contribution to the knowledge about garlic slow growth conservation at INIFAT’s Gene Bank.
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References
Boriss, H.; V. Koundinya. 2009. Garlic profile. Arizona State University. http://www.agmrc.org/commodities products/vegetables/garlic_profile.cfm: 21 de septiembre del 2009.
Burba J. L. 2008. Los grupos varietales del ajo (Allium sativum L.). Contribución para su entendimiento. Horticultura Argentina: 27(62) 20-27.
Dustan, D. I. y K. C. Short. 1977. Improved growth of tissue cultures of the onion, Allium cepa. Physiol. Plant. 41: 70-72.
ECP/GR Working group on Allium. 2006. Progress report for the period 2003-2006. http://www.ecpgr.cgiar.org/SteeringCommittee/SC10/StandRep/Allium_standrep.pdf: 21 de septiembre del 2009.
Hannan, R. M. y E. J. Sorensen 2001. Crop profile for Garlic in Washington http://www.ars.usda.gov/research/publications/publications.htm?seq_no_115=153129: 17 de marzo 2008.
Hassan, N. A.; A. A. El-Halwagi; A. Gaber; M. Al-Awady y A. Khalaf. 2007. Slow growth in vitro conservation of garlic cultivars grow in Egypt: chemical characterization and molecular evaluation. 2007. Global Journal of Molecular Sciences. 2 (2): 67-75.
Hunter, J. 2006. The medicinal Properties of Garlic . EnzineArticles.com. http://ezinearticles.com/?The-Medicinal-Properties-Of-Garlic&id=375057: 21 de septiembre del 2009.
Iannazzo, Ch. 2009. A healing herb-garlic (Allium sativum) a scientific-proven medicinal treasure trove. Herbal Medicine. Suite101.net.: 21 de septiembre del 2009.
INIFAT-MINAG, 2000. Manual Técnico de Organopónicos y Huertos Intensivos. Grupo Nacional de Agricultura Urbana. 145 p.
Izquierdo, H. y Y. Quiñones. 2001. Obtención de semilla de ajo mejorada mediante el empleo de técnicas biotecnológicas. TEMAS /septiembre –diciembre. http://www.utm.mx/temas-docs/notas15R2.pdf. 17 de marzo 2008.
Keller, J. y D.E. Lesemann. 1997. Application of in vitro culture to onion and garlic for the management and use of genetic resources at Gatersleben. Proc.I Symp. Edible Alliaceae SecIL: Burba and C.R. Galmarini Acta Hort. 433.ISHS 1997.
Lerch G. 1977. La Experimentación en las Ciencias Biológicas y Agrícolas. Editorial Científico Técnica, La Habana, 452 p.
López, R. M.; S. Pérez; L. Jiménez y R. Ronda. 2005. Influencia de la cobertura con restos de arroz en el cultivo del ajo ( Allium sativum L.) sobre surcos y canteros. Memorias VI Taller Internacional FITOGEN’ 2005, 167-168.
Materias Primas. 2009. Precios del Ajo. En Hortalizas y Verduras. Materias Primas. http://lasmateriasprimas.com/precios-del-ajo.html : 4 de mayo del 2010.
Moreno, M. A. 2009. Olvídese del oro y el petróleo, invierta en ajo. Elblogsalmon. http://www.elblogsalmon.com/economia/olvidese-del-oro-y-el-petroleo-invierta-en-ajo: 4 de mayo del 2010.
Murashige, T.; F. Skoog,. 1962. A revised meduim for rapid growth and bioessays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
Portela,J. A.; Cavagnaro J. B. 2005. escala ecofisiológica para ajos blancos y violetas: Una herramienta fundamental para la toma de decisiones en el cultivo.. Estación Experimental Agropecuaria La Consulta. Informe anual de progresos 2005. 4 pp.
Rabinowitch, H. y R. Kamenetsky. 2004. Mystery of sterility of the garlic plant solved by Hebrew University researches. Science Daily. www.sciencedaily.com/releases/2004/09/040903093636.htm: 26 de abril del 2007.
Rahman, K. 2007. Effects of garlic on platelet biochemistry and physiology. Molecular Nutrition & Food Research. 51(11) : 1335-44.
Serre M. 1996-2009. All about garlic-medicinal properties. The Worldwide Gourmet. http://www.theworldwidegourmet.com/products/articles/garlic-medicinal-properties: 25 de febrero del 2010.
Shemesh, E; O. Scholten, H. D. Rabinowitch y R. Kamenetsky. 2008. Unlocking variability: inherent variation and developmental traits of garlic plants originated from sexual reproduction. Planta.227(5):1013-1024.
Stavělíková, H. 2008. Morphologycal characteristics of garlic (Allium sativum L.)genetic resources collection-Information. Hort. Sci. (Prague) 35 (3): 130-135.
Torres, M. A. 2007. Conservación alternativa de semilla de ajo por métodos biotecnológicos. Informe Final del Proyecto 1806 del Programa Ramal Producción Nacional de Semillas. 52p.
Torres, M. A; J. Alonso; A. Font y V. Moreno. 2003 Conservación de yemas de ajo a baja temperatura. En: Caracterización, Conservación y ampliación de la variabilidad genética del germoplasma de ajo en Cuba. Informe Final del Proyecto CITMA 003181 del Programa de Biotecnología Agrícola, 30p.
